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Digestion dynamics in broilers fed rapeseed meal

Rapeseed proteins are described to be poorly digestible in chickens. To further identify some molecular locks that may limit their use in poultry nutrition, we conducted a proteomic study on the various chicken digestive contents and proposed an integrative view of the proteins recruited in the crop, proventriculus/gizzard, duodenum, jejunum, and ileum for digestion of rapeseed by-products.

Five-week-old broilers in experimental conditions. © INRA, CATTIAU Gilles
Updated on 06/13/2019
Published on 06/12/2019

Twenty-seven distinct rapeseed proteins were identified in the hydrosoluble fraction of the feed prior ingestion.
The number of rapeseed proteins identified in digestive contents decreases throughout the digestion process while some are progressively solubilized in the most distal digestive segment, likely due to a combined effect of pH and activity of specific hydrolytic enzymes. Fifteen chicken proteins were identified in the hydrosoluble proventriculus/gizzard content, including chymotrypsin-like elastase and pepsin. Interestingly, on the 69 distinct proteins identified in duodenum, only 9 were proteolytic enzymes, whereas the others were associated with homeostasis, and carbohydrate, lipid, vitamin and hormone metabolisms. In contrast, chicken proteins identified in jejunal and ileal contents were mostly proteases and peptidases.

The present work highlights the relevance of using integrative proteomics applied to the entire digestive tract to better appreciate the protein profile and functions of each digestive segment.

Contact(s)
Scientific contact(s):

Associated Division(s):
Animal Physiology and Livestock Systems
Associated Centre(s):
Val de Loire

References

Recoules, E.; Lessire, M.; Labas, V.; Duclos, M.J.; Combes-Soia, L.; Lardic, L.; Peyronnet, C.; Quinsac, A.; Narcy, A.; Rehault-Godbert, S., 2019. Digestion dynamics in broilers fed rapeseed meal. Scientific Reports, 9: 11.  http://dx.doi.org/10.1038/s41598-019-38725-1

Recoules, E.; Lessire, M.; Labas, V.; Duclos, M.J.; Combes-Soia, L.; Lardic, L.; Peyronnet, C.; Quinsac, A.; Narcy, A.; Rehault-Godbert, S., 2019. Cinétique de digestion des protéines de colza chez le poulet de chair : intérêt de la protéomique. 13ièmes JRA-JRFG, Tours, 20 & 21 March 2019.